Dartmouth logo Dartmouth College Computer Science
Technical Report series
CS home
TR home
TR search TR listserv
By author: A B C D E F G H I J K L M N O P Q R S T U V W X Y Z
By number: 2017, 2016, 2015, 2014, 2013, 2012, 2011, 2010, 2009, 2008, 2007, 2006, 2005, 2004, 2003, 2002, 2001, 2000, 1999, 1998, 1997, 1996, 1995, 1994, 1993, 1992, 1991, 1990, 1989, 1988, 1987, 1986

Complete Configuration Space Analysis for Structure Determination of Symmetric Homo-oligomers by NMR
Shobha Potluri
Dartmouth TR2008-613

Abstract: Symmetric homo-oligomers (protein complexes with similar subunits arranged symmetrically) play pivotal roles in complex biological processes such as ion transport and cellular regulation. Structure determination of these complexes is necessary in order to gain valuable insights into their mechanisms. Nuclear Magnetic Resonance (NMR) spectroscopy is an experimental technique used for structural studies of such complexes. The data available for structure determination of symmetric homo-oligomers by NMR is often sparse and ambiguous in nature, raising concerns about existing heuristic approaches for structure determination. We have developed an approach that is complete in that it identifies all consistent conformations, data-driven in that it separately evaluates the consistency of structures to data and biophysical constraints and efficient in that it avoids explicit consideration of each of the possible structures separately. By being complete, we ensure that native conformations are not missed. By being data-driven, we are able to separately quantify the information content in the data alone versus data and biophysical modeling. We take a configuration space (degree-of-freedom) approach that provides a compact representation of the conformation space and enables us to efficiently explore the space of possible conformations. This thesis demonstrates that the configuration space-based method is robust to sparsity and ambiguity in the data and enables complete, data-driven and efficient structure determination of symmetric homo-oligomers.

Note: Ph.D thesis, completed in March 2007. Filed as a tech report February 2008. Advisor: Chris Bailey-Kellogg.


PDF PDF (3324KB)

Bibliographic citation for this report: [plain text] [BIB] [BibTeX] [Refer]

Or copy and paste:
   Shobha Potluri, "Complete Configuration Space Analysis for Structure Determination of Symmetric Homo-oligomers by NMR." Dartmouth Computer Science Technical Report TR2008-613, February 2008.


Notify me about new tech reports.

Search the technical reports.

To receive paper copy of a report, by mail, send your address and the TR number to reports AT cs.dartmouth.edu


Copyright notice: The documents contained in this server are included by the contributing authors as a means to ensure timely dissemination of scholarly and technical work on a non-commercial basis. Copyright and all rights therein are maintained by the authors or by other copyright holders, notwithstanding that they have offered their works here electronically. It is understood that all persons copying this information will adhere to the terms and constraints invoked by each author's copyright. These works may not be reposted without the explicit permission of the copyright holder.

Technical reports collection maintained by David Kotz.